The media for reception callus tissues and regeneration shoot of three different sunflower genotypes was optimized. The highest shoot regeneration was observed using hypocotyl explants on MS media supplemented with 2mg/l kinetin and lmg/l NAA. Receiving of in vitro resistancecells tissues of sunflower to exometabolite of white rot (Sclerotinia sclerotiorum), on the basis of cultivation callus cells on media containing culture filtrate of fungus (Sclerotinia sclerotiorum) with different concentrations (5-35% from final volume of media). The study of the role of phenolic metabolite in the adaptation mechanisms of cell cultures to selective factors.