Protoplast fusion is a novel tool for transferring genes for a desired quality and quantity of production. In this technology, two different genetically originated protoplasts from different somatic cells are fused in order to obtain hybrid protoplasts [4]. In our case obtaining male sterile CMS carrot lines by protoplast fusion, which considered the main method used in carrot breeding. Cytoplasmic male sterility (CMS) is commercially utilized for hybrid seed production.